If you change the appearance of a Guide or Hotspot manually while working on Chrome Extension, they are considered retouches.
And, retouches can override the functioning of the theme that is designated to a Guide/Hotspot because they have programmatic superiority over themes.
That's why the best practice is to change the theme of a Guide/Hotspot rather than changing its design step by step.
If you detect any imperfection in your Guide/Hotspot design, this kind of practice could be the reason behind your issue.