In the Panel, under the Content section, there is a Hotspots Tab. Here, you can find every hotspot group you've created in the list view.
Just click on the Settings icon of any group and carry on to:
Edit the title and change its status to active or inactive
Define the target page URL via Targeting to display your hotspot group on any page(s) you want.
Define a specific user segment for the hotspot group so that your hotspot group can be displayed for any particular user group you want.
Define how frequently your hotspot group should be visible through the appearance settings.
Edit its container preference.
Finally, choose the Activate option under the Status header for the hotspots to become visible to your end-users once they arrive at the targeted URL.
If you want to edit the hotspots of any hotspot group, find its name in the Panel > Content > Hotspots and click on Open in extension so your webpage will open in a new window with UserGuiding's Extension working.