Once you have created at least one user segment through the Users Page, you can now show any of your onboarding materials to specific audiences.

Whether you're configuring a Guide, Hotspot, Checklist, etc., you will notice a section called Audience Segmentation. There, you'll choose the Custom Segment option and the segment you've created.
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In the example below, we have decided to show the content only to our trial users.

Note: You can add multiple segments and combine them with AND/OR to customize your segmentation even more.